Tayebeh Rastegar
Tehran University of Medical Sciences, IranTitle: Pentoxifylline promotes spermatogenesis via upregulation of the Nrf2-ARE signalling pathway in a mouse model of germ-cell apoptosis induced by testicular torsion–detorsion
Abstract
Context. Testicular torsion–detorsion results in loss of germ cells and infertility. Pentoxifylline has been shown to prevent tissue damage.
Aims. To determine the effect of pentoxifylline on germ cell survival in torsion–detorsion induced apoptosis
Methods. Twenty male mice were divided into four groups of five animals each: Control, T1 (Torsion–detorsion + single dose 100 mg/kg Pentoxifylline/IP), T2 (Torsion–detorsion + daily 20 mg/kg pentoxifylline/IP for 2 weeks, and T/D (Torsion–detorsion only). 35th day after torsion–detorsion, the left testes of all the animals were harvested for histological and biochemical analysis.
Key results. Histomorpholoical analysis showed significant increase (P < 0.05) in seminiferous tubule diameter, Johnsen’s score and germ cells of Control and T1 compared to T2 and T/D, with no significant difference (P > 0.05) in testis weight, sertoli, leydig and myoid cells. Tunnel assay showed significant increase (P < 0.05) in apoptotic cells of T/D and T2 animals compared to Control and T1. RT-PCR analysis showed significant high (P < 0.01) mRNA expression of Bax gene in T/D compared to T1 and T2 and significant increase (P < 0.05) of Bcl2 in Control, T1, T2 compared to T/D. Nrf2-ARE transcripts revealed significant increase (P < 0.05) in Control and T1 compared to T2 and T/D. Western blot showed significantly increased (P < 0.05) caspase-3 in T/D compared to Control, T1 and T2.
Conclusion. Pentoxifylline promotes spermatogenesis and suppressed apoptosis induced by testicular torsion–detorsion.
Implication. Pentoxifylline could serve as adjunct therapy to surgery in the treatment of torsion–detorsion induced germ cell apoptosis
Biography
Tayebeh Rastegar, an associate professor in the anatomy department of the Faculty of Medicine, graduated at PhD in Anatomical Sciences from Tehran University of Medical Sciences in 2013, and in 2013 started serving as a faculty member in this university. She involved in teaching of Anatomy, Histology, embryology and histological techniques. It should be noted that since the establishment of the International Vice-Chancellor and the recruitment of foreign students, she has also collaborated in teaching the following courses in English.
She has published more than 60 research articles about male reproductive biology, stem cells differentiation and transplantation and …., Also has been serving as an editorial board member of journal on Anatomy forecast
Organization Location
1309 Coffeen Avenue, STE 1200, Sheridan, Wyoming 82801, USA
Mail us at
Program Enquiry
midwifery@scientexconferences.com
General Queries
contact@scientexconferences.com
Registration Enquiry
finance@scientexconferences.com
neonatalcare@scientexevents.com
Our Social Presence
Call us at
USA :+1 (689) 267 3738